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ALL IHFOEHATIOH COOTAIIIED 

HEREIU IS UNCLASSIFIED 

DATE 12-16-2008 BY 60324 UC BAW/DK/P.YS 



U.S. Department of Justice 


Federal Bureau of Investigation 


In Reply, Please Refer to 
File No. 


August 24, 2004 






^rter Street 


Fort Detrick, Maryland 21702 


Dear 


Pursuant to the ongoing criminal investigation into the 
2001 mailings of letters laced with Bacillus anthracis, the 
Federal Bureau of Investigation (FBI) requests the items, 
documents, and/or access listed below. If a point of contact for 
an item or document has been identified by one of our 
investigators, that point of contact is _ listed with the item or 
docum ent . Please contact Special Agent 


when the items are available for pickup from USAMRIID. 


Any electron microscopy stubs and mi crographs from an y 
Ames spore preparations at USAMRIID 



Final contract documents from the contract between 
USAMRIID and .Du^ay for the production of Ames spores 
for aerosol chadlSTges including the protocol actually 
used, micrographs, and other documentation (Bruce 
Ivins) 


Requirements for the production of Standard 
Materials in the Bacteriology Divis'ion ~ 


.eference. 


Research Protocols numbered BOl-07, BOO-03, D98-03, 
FOO-11, D99-02 for aerosol challenges. 


Access to personnel records for the following 
employees : 




(< 




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Travel, leave, and time and attendance records for the 
following employees: 



■ 


Laboratory notes, computer files, and other 
documentation regarding Ames samples, Ames research and 
Ames spo re production _kenL_tiVJ 


1 Rriice Tvins.r 


Frederick 


Knauert (deceased^ 


Oliver Mil^ esell (deceased) , 
Several of these 


individuals are no longer at USAMRIID, but their 
documentation was left at USAMRIID. 


2 


Sincerely, 


Inspector in Charge 

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Byi 


supervisory apeciai Agent 


cc: 


1 - Addressee 

^ -I I . 

2 - 279A-WF-222936-USAMRIID 
1 - work copy 

15i . 


3 


(h A 

- FD-302 (Rev. 10-6-95) 




- 1 - 

FEDERAL BUREAU OF INVESTIGATION 


ALL IHFORMATIOH COHTAniELi 
HEREIH IS UMCLASSiriEI) 

DATE 12-16-2008 BY 60324 UC BAW/DK./RYS 


pn July 17-19, 2004, 


Date of transcription 07 /30/2004 

date of birth 


Social Security Account Numberl 


at l I place of employment, the 
United States Army Medical Research Institute of Infectious 
Diseases (USAMRIID) , Fort Detrick, Frederi ck, Maryland. SA 


conducted the search 


for a nd in ven tory of Bacillus anthracis ( B. a. ) ma intained in 
H and 


roomsL 


of USAMRIID 's Building 


SA 


Decroit Division, was also present on July 1 / , 2004. 


provided the following information wi th resp ect 
to items located in the glass-door refrigerator in room 
[sample container notations are denoted below in bold ] : 


The 1.5 milliliter (mL) vial labeled Ames 


1 Bargee's contained ~1 mL of an Ames spore suspension. 


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The 1 liter (L) flask labeled 2003 

Bruce Ivins contained -550 mL of an Ames 


spore s uspension. The spores were p roduced at Dugway Proving 
] and were purified bv IVINS. f 



The 0.5 mL vial labeled 


DPG spores 


Investigation on 07/17/2004 at Frederick, Maryland 


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This document contains neither recommendations nor conclusions of the FBI. It is the property of the FBI and is loaned to your agency; 
it and Its contents are not to be distributed outside your agency. 


cr cr 


^ FD-302a (Rev. 10-6-95) 




279A-WF-222936-USAMRIID 


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Continuation of FD-302 of 


■ On 07/17/2004 .Page 2_ 



DO 

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The 0 ■ 5 mL vial labeled 


contained 



The 1.25 mL vial labeled Ducr wav Ames spores, 1 x 10^°/mL 


contained ~1 mL of a spore suspension. f 


indicated that 


I I likely received this spore material from IVINS . 



FD-302a(Rev. 10-6-95) 


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provided no additional i nformation with regard 


to the 1 . 5 mL vial labeled 


did not recall the 


Ames stock used to generate the spores, nor did| |recall the 
purpose of the material. 


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The :j 

> mL tube ] 
contained 

■abeled 



1 r~ 





The small test tube labeled ' I 

I I from Bruce Ivins I I 



FD-302a (Rev. 10-6-95) 


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Continuation of FD-302 of 


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The 1.25 mL vial labeled . 


1 contained 


Accordina to | 

1 this material was 



submitted previously to the FBI Repository. 



The 250 mL flask labeled ] | 

contained 


The 1 mL vial labeled 


contained 
]did not know the origin of 


this material, nor the identity of the B.a. strain. 


FD-302a (Rev. 10-6-95) 


2 7 9 A-WF -2 2 2 9 3 6 “USMCRI ID 


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Continuation of FD-302 of 


, On 07 / 17 72004 , Page , 


During the inventory of the room 
quality control samples related to weeks [ 


refr igerator , 


of 


study were located. The "QC" samples deriv ed from a blind study 


identify the materials by B.a. strain. 





in the roomf 


F ollow ing is a lis t of the eight "QC" samples presen t 



On July 21, 2002, SA 


viewed information 


contained in email exchanges among USAMRIID personnel, to 


regarding the ident i 
the information froni 
itiessaaes indicated t 

ties of the 

saI 

viewed 

email 

1 computer in room , The 

hatl 



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1 

1. From the information provided, it 

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was determined that the I 

located in the room 

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. ^ Ames. According to the email 

document ation, p .a. Ames was used during test weeld I and was 
coded as [ 


inventory of room 
exchanges r elated to the 


No such mater ial was located during the 

provid ed a hard copy of the email 
I to Sa[ 


on 


The refri gerator in room I [ contained multi ple item s 

which, according to I were samples generated by [ 

I at the reques t of the FBI. Aliquots of 
unese maceriaxs were not collectef^-l ~lcorifirmed that the 


by 


prohuceq by 

1 Th e material was ge nerated from an Am es stock provided 


and was used for 


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provided the f ollowing information wi th respect b6 


to materials located in the room 
container notations are denoted below in bold ] : 


[sample 


The 50 mL conical tube 1 ad Lot 

(Bruce Ivins) from 


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1 contained f 


gpnprstp.f^ the .qpnrR.q from IVINS ' 


[indicated that the FBI di d not take this Ames 

material dur ing a previous co llection of [ spore materials 
stored in the | Additionally, the tube was not 
fingerprinted, as were the othe r tubes con taining Ames spore 
prepar ations stored by in the room 


contained 


The 1. 25 m il vial labeledf 


of 


which Ames stock was used to generate these spores. 


J did not recall 


Four items were located in the room 
room in a box labeled [ 


items were associated with research conducted by 
and personnel working with 


] indi cated that the 


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] 


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FD-302a (Rev. 10-6-95) 



279A-WF-222936-USAMRIID 


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Continuation of FD-302 of 


On 07 / 17 / 2004 , Page 


employed at USM CIIID. The 1.25 mL vial labeled [ 


1 contained 1 

The 1.25 

mL vial 

labeledl 1 

contained 


1 'me i.A'd mb vial labeledl 

containedi 

1 A nt tour 


identical vials of "B.a. Ames 

were present . 


believed that the spores identified as 1 


land ' 

Iwere 

generated by 



The informat ion pr ovide d bvj 
of USAMRiID's Building 


and 


during the searches 
was noted on 


rooms 

inventory log sheets . The inventory log sheets are maintained in 
a lA envelope (FD-340) , along with additional documents related 
to the USAMRIID search conducted on July 16-23, 2004. 


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